Formulation and Evaluation of Letrozole Nanoparticles by Salting Out Technique and Determination of Anti-cancer Activity by MTT Assay

Objective Letrozole (LTZ) drug is an aromatase inhibitor used for the treatment of hormonally positive breast cancer in postmenopausal women. It has poor water solubility, rapid metabolism and a range side effects. In this study, polymer-based nanoparticles (NPs) incorporating have been formulated evaluated, aimed to control release, potentially maximize therapeutic efficiency, minimize effects drug. Methodology The incorporated into polymer (i.e., Eudragit S 100 Ethyl cellulose) by employing salting out technique. Total twelve formulations were prepared varying drug-polymer concentrations organic aqueous phase ratios evaluated percentage yield, content invitro release studies. Out 12 formulations, best selected based on studies characterized mean particle diameter zeta potential. Results Among all F5EC, 1:2 was considered be formulation with minimum size 194.55 nm, potential value –18.6 mV, 90.28%, entrapment efficiency 92.36%, 95% within h. kinetic indicated that followed zero order kinetics showed non-fickian diffusion mechanism. Based evaluation characterization technique (F5EC 1:2) determination anti-cancer activity MCF-7 cell line MTT assay. results exhibited IC50 49.63 ng. Conclusion Finally, comparing results, cellulose (EC) most suitable preparation LTZ NPs Entrapment Efficiency improved up 92.36% using